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| Kratz,J.M.; Teixeira,M.R.; Koester,L.S.; Simões,C.M.O.. |
| The Caco-2 cell line has been used as a model to predict the in vitro permeability of the human intestinal barrier. The predictive potential of the assay relies on an appropriate in-house validation of the method. The objective of the present study was to develop a single HPLC-UV method for the identification and quantitation of marker drugs and to determine the suitability of the Caco-2 cell permeability assay. A simple chromatographic method was developed for the simultaneous determination of both passively (propranolol, carbamazepine, acyclovir, and hydrochlorothiazide) and actively transported drugs (vinblastine and verapamil). Separation was achieved on a C18 column with step-gradient elution (acetonitrile and aqueous solution of ammonium acetate, pH... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caco-2; HPLC-UV; Marker drugs; Permeability. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000600006 |
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| FELICIO,ANA LUCIA S.M.; MONTEIRO,ALESSANDRA M.; ALMEIDA,MARIANA B.; MADEIRA,TIAGO B.; NIXDORF,SUZANA L.; YABE,MARIA JOSEFA S.. |
| ABSTRACT Diuron is one of the most widely herbicide used worldwide, which can undergo degradation producing three primary metabolites: 3,4-dichlorophenylurea, 3-(3,4-dichlorophenyl)-1-methylurea, and 3,4-dichloroaniline. Since the persistence of diuron and its by-products in ecosystems involves risk of toxicity to environment and human health, a reliable quantitative method for simultaneous monitoring of these compounds is required. Hence, a simple method without preconcentration step was validated for quantitation of diuron and its main metabolites by high performance liquid chromatography with ultraviolet detection. Separation was achieved in less than 11 minutes using a C18 column, mobile phase composed of acetonitrile and water (45:55 v/v) at 0.86 mL... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pesticides; Degradation products; HPLC-UV; Soil; Environmental control. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401235 |
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| Assis,D.C.S.; Menezes,L.D.M.; Lima,A.L.; Klein,R.W.T.; Heneine,L.G.D.; Lara,L.J.C.; Teixeira,L.V.; Ornellas,C.B.D.; Cançado,S.V.; Figueiredo,T.C.. |
| ABSTRACT A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was validated for the study of bioactive amines in chicken meat. A gradient elution system with an ultraviolet detector was used after extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Putrescine, cadaverine, histamine, tyramine, spermidine, and spermine standards were used for the evaluation of the following performance parameters: selectivity, linearity, precision, recovery, limits of detection, limits of quantification and ruggedness. The results indicated excellent selectivity, separation of all amines, a coefficient of determination greater than 0.99 and recovery from 92.25 to 102.25% at the concentration of 47.2mg.kg-1,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Validation; Chicken meat; Bioactive amines; HPLC-UV. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300805 |
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